Derivatization in Analytical Chemistry
Derivatization_in_Analytical_Chemistry
Source: http://www.mdpi.com/books
Edited by: Paraskevas D. Tzanavaras
Abstract: Herein, we report a new automated flow method based on zone fluidics for the simultaneous determination of homocysteine and homocysteine thiolactone using fluorimetric detection (lext = 370 nm/lem = 480 nm). Homocysteine thiolactone is hydrolyzed on-line in alkaline medium (1 mol L−1 NaOH) to yield homocysteine, followed by reaction with o-phthalaldehyde in a single step.
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