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Effects of Heavy Metals on Cell Density, Size, Specific Growth Rate and Chlorophyll a of Tetraselmis Tetrathele Under Controlled Laboratory Conditions
Abstract
The effects of the varying levels of mercury (Hg) and cadmium (Cd) (0, 0.1, 0.3, 0.5, 1.0, 2.0, 3.0, 4.0 and 5.0 mg L⁻¹) to the cellular density of the green microalgae Tetraselmis tetrathele were evaluated every 24 h for 120 h. Specific growth rate, cell sizes and chlorophyll a were also monitored in the 5.0 mg L⁻¹ Hg and Cd and were compared to the unexposed at 0, 12, 24, 36, 48 and 120h. Results showed that the algal density of T. tetrathele exposed to various levels of Hg were similar with the control up to 48 h. Variations on different concentrations at different times were observed but the results suggest that T. tetrathele was not affected by Hg even at concentrations up to 5.0 mg L⁻¹ for 48 h but started to show toxicity from 3.0 to 5.0 mg L⁻¹ after 72 h and longer. Cd on the other hand also showed toxicity at 3.0, 4.0 and 5.0 mg L⁻¹ beyond 24 h exposure. The specific growth rate of T. tetrathele exposed to both 5.0 mg L⁻¹ Hg and Cd was statistically similar with those of the unexposed from 0 to 12 h and negative growth rates then followed up to 36 h. The chlorophyll a was significantly lower in the metal -exposed algae than did those unexposed. Chlorophyll a also decreased in T. tetrathele exposed to both heavy metals but algal cell sizes were not affected with the presence of Hg or Cd in the culture system.
Effects of Heavy Metals on Cell Density, Size, Specific Growth Rate and Chlorophyll a of Tetraselmis Tetrathele Under Controlled Laboratory Conditions
Abstract
The effects of the varying levels of mercury (Hg) and cadmium (Cd) (0, 0.1, 0.3, 0.5, 1.0, 2.0, 3.0, 4.0 and 5.0 mg L⁻¹) to the cellular density of the green microalgae Tetraselmis tetrathele were evaluated every 24 h for 120 h. Specific growth rate, cell sizes and chlorophyll a were also monitored in the 5.0 mg L⁻¹ Hg and Cd and were compared to the unexposed at 0, 12, 24, 36, 48 and 120h. Results showed that the algal density of T. tetrathele exposed to various levels of Hg were similar with the control up to 48 h. Variations on different concentrations at different times were observed but the results suggest that T. tetrathele was not affected by Hg even at concentrations up to 5.0 mg L⁻¹ for 48 h but started to show toxicity from 3.0 to 5.0 mg L⁻¹ after 72 h and longer. Cd on the other hand also showed toxicity at 3.0, 4.0 and 5.0 mg L⁻¹ beyond 24 h exposure. The specific growth rate of T. tetrathele exposed to both 5.0 mg L⁻¹ Hg and Cd was statistically similar with those of the unexposed from 0 to 12 h and negative growth rates then followed up to 36 h. The chlorophyll a was significantly lower in the metal -exposed algae than did those unexposed. Chlorophyll a also decreased in T. tetrathele exposed to both heavy metals but algal cell sizes were not affected with the presence of Hg or Cd in the culture system.
Basic Laboratory Study Guide
Preface:
The Laboratory Study Guide is an important resource for preparing for the certification exam and is arranged by chapters and sections. Each section consists of key knowledges with important informational concepts you need to know for the certification exam. This study guide also serves as a wastewater treatment plant operations primer that can be used as a reference on the subject. In preparing for the exams:
- Study the material! Read every key knowledge until the concept is fully understood and knownto memory.
- Learn with others! Take classes in this type of wastewater operations to improve your understanding and knowledge of the subject.
- Learn even more! For an even greater understanding and knowledge of the subjects, read and review the references listed at the end of the study guide.
Basic Laboratory Study Guide
Preface:
The Laboratory Study Guide is an important resource for preparing for the certification exam and is arranged by chapters and sections. Each section consists of key knowledges with important informational concepts you need to know for the certification exam. This study guide also serves as a wastewater treatment plant operations primer that can be used as a reference on the subject. In preparing for the exams:
- Study the material! Read every key knowledge until the concept is fully understood and knownto memory.
- Learn with others! Take classes in this type of wastewater operations to improve your understanding and knowledge of the subject.
- Learn even more! For an even greater understanding and knowledge of the subjects, read and review the references listed at the end of the study guide.
Analysis of Wastewater for Use in Agriculture – A Laboratory Manual of Parasitological and Bacteriological Techniques
Introduction:
The use of wastewater for crop irrigation is becoming increasingly common, especially in arid and semi-arid areas. Crop yields are higher as the wastewater contains not only water for crop growth, but also plant nutrients (mainly nitrogen and phosphorus). However, there is the risk that wastewater irrigation may facilitate the transmission of excreta-related diseases. In the late 1980s, the World Health Organization, the World Bank and the International Reference Centre for Waste Disposal sponsored a series of studies and meetings of experts to examine these health risks (International Reference Centre for Waste Disposal, 1985; Shuval et al., 1986; Prost, 1988; World Health Organization, 1989). From an appraisal of the available epidemiological evidence, it was established that the major risks were: the transmission of intestinal nematode infections both to those working in the waste-water irrigated fields and to those consuming vegetables grown in the fields; these infections are due to Ascaris lumbricoides (the human roundworm), Trichuris trichiura (the human whipworm), and Ancylostoma duodenale and Necator americanus (the human hookworms); and- the transmission of faecal bacterial diseases - bacterial diarrhoea and dysentery, typhoid and cholera - to the crop consumers.
Analysis of Wastewater for Use in Agriculture – A Laboratory Manual of Parasitological and Bacteriological Techniques
Introduction:
The use of wastewater for crop irrigation is becoming increasingly common, especially in arid and semi-arid areas. Crop yields are higher as the wastewater contains not only water for crop growth, but also plant nutrients (mainly nitrogen and phosphorus). However, there is the risk that wastewater irrigation may facilitate the transmission of excreta-related diseases. In the late 1980s, the World Health Organization, the World Bank and the International Reference Centre for Waste Disposal sponsored a series of studies and meetings of experts to examine these health risks (International Reference Centre for Waste Disposal, 1985; Shuval et al., 1986; Prost, 1988; World Health Organization, 1989). From an appraisal of the available epidemiological evidence, it was established that the major risks were: the transmission of intestinal nematode infections both to those working in the waste-water irrigated fields and to those consuming vegetables grown in the fields; these infections are due to Ascaris lumbricoides (the human roundworm), Trichuris trichiura (the human whipworm), and Ancylostoma duodenale and Necator americanus (the human hookworms); and- the transmission of faecal bacterial diseases - bacterial diarrhoea and dysentery, typhoid and cholera - to the crop consumers.
Investigation of The Quality of Water Treated by Magnetic Fields
Abstract
Passing water through a magnetic field has been claimed to improve chemical, physical and bacteriological quality of water in many different applications. Although the treatment process has been used for decades, it still remains in the realms of pseudoscience. If the claims of treating water with magnets are true, the process offers improvements on many of our applications of water in today’s world. A large number of peer reviewed journal articles have reported contradictory claims about the treatment.. Some of the most beneficial claimed water applications from magnetically treated water include improvement in scale reduction in pipes and enhanced crop yields with reduced water usage. Today we are still unsure whether the technology works and those who do believe it works are still trying to understand the mechanisms of how it works. Many research papers are starting to develop similar theories behind the mechanism of the treatment. From previous studies, it has been determined that the most successful MTD’s are those with alternating poles. The majority of the experiments performed during this research were determined to have insufficient controls to produce conclusive results. The conclusions from this research were focused on designing improved experiments to provide more conclusive results. A theory was developed to explain the MTD’s mechanisms of scale reduction. While the experimental results were not conclusive, the results attained backed the theory. Magnetically treated water does not do all that it is claimed it does. However, some of the positive results obtained during this research suggest that the improved experiments developed from this research may provide conclusive results on this controversial topic.
Investigation of The Quality of Water Treated by Magnetic Fields
Abstract
Passing water through a magnetic field has been claimed to improve chemical, physical and bacteriological quality of water in many different applications. Although the treatment process has been used for decades, it still remains in the realms of pseudoscience. If the claims of treating water with magnets are true, the process offers improvements on many of our applications of water in today’s world. A large number of peer reviewed journal articles have reported contradictory claims about the treatment.. Some of the most beneficial claimed water applications from magnetically treated water include improvement in scale reduction in pipes and enhanced crop yields with reduced water usage. Today we are still unsure whether the technology works and those who do believe it works are still trying to understand the mechanisms of how it works. Many research papers are starting to develop similar theories behind the mechanism of the treatment. From previous studies, it has been determined that the most successful MTD’s are those with alternating poles. The majority of the experiments performed during this research were determined to have insufficient controls to produce conclusive results. The conclusions from this research were focused on designing improved experiments to provide more conclusive results. A theory was developed to explain the MTD’s mechanisms of scale reduction. While the experimental results were not conclusive, the results attained backed the theory. Magnetically treated water does not do all that it is claimed it does. However, some of the positive results obtained during this research suggest that the improved experiments developed from this research may provide conclusive results on this controversial topic.
Clearing the Waters A focus on water quality solutions
Reproduction
This publication may be reproduced in whole or in part and in any form for educational or nonprofit purposes without special permission from the copyright holders, provided acknowledgement of the source is made. UNEP would appreciate receiving a copy of any publication that uses this publication as a source. No use of this publication may be made for resale or for any other commercial purpose whatsoever without prior permission in writing from the United Nations Environment Programme.
Clearing the Waters A focus on water quality solutions
Reproduction
This publication may be reproduced in whole or in part and in any form for educational or nonprofit purposes without special permission from the copyright holders, provided acknowledgement of the source is made. UNEP would appreciate receiving a copy of any publication that uses this publication as a source. No use of this publication may be made for resale or for any other commercial purpose whatsoever without prior permission in writing from the United Nations Environment Programme.
Developing Quality Control Handbooks for Water and Wastewater Treatment
Introduction
This section provides a step by step discussion regarding the development of a Quality Control Handbook for a water or wastewater treatment plant. A workshop format has been chosen for the development of the handbook in order to channel the multiple knowledge inputs needed for it. For a proper. The workshop format targets participants coming from water or wastewater treatment facilities. These participants are expected to be knowledgeable of the operational activities of the plant and able to share their actual experiences. The successful implementation of this handbook depends on the creation of a setting in which the employees themselves act as both teachers and students. Active participation is a key success factor, and practical demonstrations are necessary to supplement the theoretical aspects. The workshop format can be easily replicated and as such, helps facilitate the development of operating manuals in other water treatment facilities or water companies. Once completed, these manuals may be used to define the required skills of the operating personnel and may also be used for actual on-the-job training. The handbook is to be finalized after successful completion of four group sessions.
Developing Quality Control Handbooks for Water and Wastewater Treatment
Introduction
This section provides a step by step discussion regarding the development of a Quality Control Handbook for a water or wastewater treatment plant. A workshop format has been chosen for the development of the handbook in order to channel the multiple knowledge inputs needed for it. For a proper. The workshop format targets participants coming from water or wastewater treatment facilities. These participants are expected to be knowledgeable of the operational activities of the plant and able to share their actual experiences. The successful implementation of this handbook depends on the creation of a setting in which the employees themselves act as both teachers and students. Active participation is a key success factor, and practical demonstrations are necessary to supplement the theoretical aspects. The workshop format can be easily replicated and as such, helps facilitate the development of operating manuals in other water treatment facilities or water companies. Once completed, these manuals may be used to define the required skills of the operating personnel and may also be used for actual on-the-job training. The handbook is to be finalized after successful completion of four group sessions.
Boiler Water Quality Requirements and Associated Steam Quality for Industrial/Commercial and Institutional Boilers
Introduction:
The purpose of this publication is to acquaint engineers, purchasers and operators of industrial, commercial and institutional (ICI) boilers with ABMA's judgment as to the relationship between boiler water quality and boiler performance. This document is published for general guidance as a supplement to detailed operating manuals supplied by the equipment manufacturers. It should also be noted that the information presented is directed to steel boiler designs, as opposed to cast iron sectional or copper finned tube boilers. Furthermore Utility Boilers and Combined Cycle Boilers, which require extremely close control of water quality and steam purity, are not the topic of this document. This new document combines two previous ABMA Guideline documents, namely “Boiler Water Requirements and Associated Steam Purity for Commercial Boilers” (1998), and “Boiler Water Limits and Achievable Steam Purity for Water tube Boilers”, (1995). The document discusses the effect of various feed water and condensate systems on the boiler operation. It also provides information on boiler water and steam testing as well as system care and maintenance. It is recognized that specific boiler usage and water treatment will vary and may require values different from these recommendations. Boiler users therefore, need to define limits, equipment and operating parameters for their particular application. These recommendations are for information only. Everyone is free to accept or reject the conclusions of these suggestions as their own judgment warrants in all aspects of the conduct of their business. The ABMA does not represent or warrant that any level of steam purity depicted will be achieved by any particular boiler or boilers.
Boiler Water Quality Requirements and Associated Steam Quality for Industrial/Commercial and Institutional Boilers
Introduction:
The purpose of this publication is to acquaint engineers, purchasers and operators of industrial, commercial and institutional (ICI) boilers with ABMA's judgment as to the relationship between boiler water quality and boiler performance. This document is published for general guidance as a supplement to detailed operating manuals supplied by the equipment manufacturers. It should also be noted that the information presented is directed to steel boiler designs, as opposed to cast iron sectional or copper finned tube boilers. Furthermore Utility Boilers and Combined Cycle Boilers, which require extremely close control of water quality and steam purity, are not the topic of this document. This new document combines two previous ABMA Guideline documents, namely “Boiler Water Requirements and Associated Steam Purity for Commercial Boilers” (1998), and “Boiler Water Limits and Achievable Steam Purity for Water tube Boilers”, (1995). The document discusses the effect of various feed water and condensate systems on the boiler operation. It also provides information on boiler water and steam testing as well as system care and maintenance. It is recognized that specific boiler usage and water treatment will vary and may require values different from these recommendations. Boiler users therefore, need to define limits, equipment and operating parameters for their particular application. These recommendations are for information only. Everyone is free to accept or reject the conclusions of these suggestions as their own judgment warrants in all aspects of the conduct of their business. The ABMA does not represent or warrant that any level of steam purity depicted will be achieved by any particular boiler or boilers.
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