The Theory of HPLC (Column Chemistry)
Source: https://www.chromacademy.com
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Electrical & Automation
Aims and Objectives
Aims
To introduce silica as a major stationary phase support for Reverse Phase HPLC separations and for Normal Phase applications
To explain how bonded phases are produced and to highlight the deactivation process used
To explain the use of bonded phases in various chromatographic applications and the influence of surface silica silanol groups on chromatographic separations
To introduce modern bonded phases that give an advantage to today’s chromatographer – including water wettable phases, Polar Embedded phase and Phase capable of operating at extremes of pH
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