The Theory of HPLC (Column Chemistry)


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Aims and Objectives
Aims
 To introduce silica as a major stationary phase support for Reverse Phase HPLC separations and for Normal Phase applications
 To explain how bonded phases are produced and to highlight the deactivation process used
 To explain the use of bonded phases in various chromatographic applications and the influence of surface silica silanol groups on chromatographic separations
 To introduce modern bonded phases that give an advantage to today’s chromatographer – including water wettable phases, Polar Embedded phase and Phase capable of operating at extremes of pH

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