Laboratory & Water Quality
An Introduction to Water Quality Monitoring
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Analysis of Wastewater for Use in Agriculture – A Laboratory Manual of Parasitological and Bacteriological Techniques
Introduction:
The use of wastewater for crop irrigation is becoming increasingly common, especially in arid and semi-arid areas. Crop yields are higher as the wastewater contains not only water for crop growth, but also plant nutrients (mainly nitrogen and phosphorus). However, there is the risk that wastewater irrigation may facilitate the transmission of excreta-related diseases. In the late 1980s, the World Health Organization, the World Bank and the International Reference Centre for Waste Disposal sponsored a series of studies and meetings of experts to examine these health risks (International Reference Centre for Waste Disposal, 1985; Shuval et al., 1986; Prost, 1988; World Health Organization, 1989). From an appraisal of the available epidemiological evidence, it was established that the major risks were: the transmission of intestinal nematode infections both to those working in the waste-water irrigated fields and to those consuming vegetables grown in the fields; these infections are due to Ascaris lumbricoides (the human roundworm), Trichuris trichiura (the human whipworm), and Ancylostoma duodenale and Necator americanus (the human hookworms); and- the transmission of faecal bacterial diseases - bacterial diarrhoea and dysentery, typhoid and cholera - to the crop consumers.
Analysis of Wastewater for Use in Agriculture – A Laboratory Manual of Parasitological and Bacteriological Techniques
Introduction:
The use of wastewater for crop irrigation is becoming increasingly common, especially in arid and semi-arid areas. Crop yields are higher as the wastewater contains not only water for crop growth, but also plant nutrients (mainly nitrogen and phosphorus). However, there is the risk that wastewater irrigation may facilitate the transmission of excreta-related diseases. In the late 1980s, the World Health Organization, the World Bank and the International Reference Centre for Waste Disposal sponsored a series of studies and meetings of experts to examine these health risks (International Reference Centre for Waste Disposal, 1985; Shuval et al., 1986; Prost, 1988; World Health Organization, 1989). From an appraisal of the available epidemiological evidence, it was established that the major risks were: the transmission of intestinal nematode infections both to those working in the waste-water irrigated fields and to those consuming vegetables grown in the fields; these infections are due to Ascaris lumbricoides (the human roundworm), Trichuris trichiura (the human whipworm), and Ancylostoma duodenale and Necator americanus (the human hookworms); and- the transmission of faecal bacterial diseases - bacterial diarrhoea and dysentery, typhoid and cholera - to the crop consumers.
Effects of Heavy Metals on Cell Density, Size, Specific Growth Rate and Chlorophyll a of Tetraselmis Tetrathele Under Controlled Laboratory Conditions
Abstract
The effects of the varying levels of mercury (Hg) and cadmium (Cd) (0, 0.1, 0.3, 0.5, 1.0, 2.0, 3.0, 4.0 and 5.0 mg L⁻¹) to the cellular density of the green microalgae Tetraselmis tetrathele were evaluated every 24 h for 120 h. Specific growth rate, cell sizes and chlorophyll a were also monitored in the 5.0 mg L⁻¹ Hg and Cd and were compared to the unexposed at 0, 12, 24, 36, 48 and 120h. Results showed that the algal density of T. tetrathele exposed to various levels of Hg were similar with the control up to 48 h. Variations on different concentrations at different times were observed but the results suggest that T. tetrathele was not affected by Hg even at concentrations up to 5.0 mg L⁻¹ for 48 h but started to show toxicity from 3.0 to 5.0 mg L⁻¹ after 72 h and longer. Cd on the other hand also showed toxicity at 3.0, 4.0 and 5.0 mg L⁻¹ beyond 24 h exposure. The specific growth rate of T. tetrathele exposed to both 5.0 mg L⁻¹ Hg and Cd was statistically similar with those of the unexposed from 0 to 12 h and negative growth rates then followed up to 36 h. The chlorophyll a was significantly lower in the metal -exposed algae than did those unexposed. Chlorophyll a also decreased in T. tetrathele exposed to both heavy metals but algal cell sizes were not affected with the presence of Hg or Cd in the culture system.
Effects of Heavy Metals on Cell Density, Size, Specific Growth Rate and Chlorophyll a of Tetraselmis Tetrathele Under Controlled Laboratory Conditions
Abstract
The effects of the varying levels of mercury (Hg) and cadmium (Cd) (0, 0.1, 0.3, 0.5, 1.0, 2.0, 3.0, 4.0 and 5.0 mg L⁻¹) to the cellular density of the green microalgae Tetraselmis tetrathele were evaluated every 24 h for 120 h. Specific growth rate, cell sizes and chlorophyll a were also monitored in the 5.0 mg L⁻¹ Hg and Cd and were compared to the unexposed at 0, 12, 24, 36, 48 and 120h. Results showed that the algal density of T. tetrathele exposed to various levels of Hg were similar with the control up to 48 h. Variations on different concentrations at different times were observed but the results suggest that T. tetrathele was not affected by Hg even at concentrations up to 5.0 mg L⁻¹ for 48 h but started to show toxicity from 3.0 to 5.0 mg L⁻¹ after 72 h and longer. Cd on the other hand also showed toxicity at 3.0, 4.0 and 5.0 mg L⁻¹ beyond 24 h exposure. The specific growth rate of T. tetrathele exposed to both 5.0 mg L⁻¹ Hg and Cd was statistically similar with those of the unexposed from 0 to 12 h and negative growth rates then followed up to 36 h. The chlorophyll a was significantly lower in the metal -exposed algae than did those unexposed. Chlorophyll a also decreased in T. tetrathele exposed to both heavy metals but algal cell sizes were not affected with the presence of Hg or Cd in the culture system.
Basic Laboratory Study Guide
Preface:
The Laboratory Study Guide is an important resource for preparing for the certification exam and is arranged by chapters and sections. Each section consists of key knowledges with important informational concepts you need to know for the certification exam. This study guide also serves as a wastewater treatment plant operations primer that can be used as a reference on the subject. In preparing for the exams:
- Study the material! Read every key knowledge until the concept is fully understood and knownto memory.
- Learn with others! Take classes in this type of wastewater operations to improve your understanding and knowledge of the subject.
- Learn even more! For an even greater understanding and knowledge of the subjects, read and review the references listed at the end of the study guide.
Basic Laboratory Study Guide
Preface:
The Laboratory Study Guide is an important resource for preparing for the certification exam and is arranged by chapters and sections. Each section consists of key knowledges with important informational concepts you need to know for the certification exam. This study guide also serves as a wastewater treatment plant operations primer that can be used as a reference on the subject. In preparing for the exams:
- Study the material! Read every key knowledge until the concept is fully understood and knownto memory.
- Learn with others! Take classes in this type of wastewater operations to improve your understanding and knowledge of the subject.
- Learn even more! For an even greater understanding and knowledge of the subjects, read and review the references listed at the end of the study guide.
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